Genetic Alteration
Genetic Alteration - Related Videos
11:02
Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
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Cited by 64 •
2013
We describe the preparation of barcoded DNA libraries and subsequent hybridization-based exon capture for detection of key cancer-associated mutations in clinical tumor specimens by massively parallel "next generation" sequencing. Targeted exon sequencing offers the benefits of high throughput, low cost, and deep sequence coverage, thus yielding high sensitivity for detecting low frequency mutations.
09:03
Forward Genetic Approaches in Chlamydia trachomatis
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Cited by 21 •
2013
We describe a methodology to perform genetic analysis in Chlamydia based on chemical mutagenesis and whole genome sequencing. In addition, a system for DNA exchange within infected cells is described that can be used for genetic mapping. This method may be broadly applicable to microbial systems lacking transformation systems and molecular genetic tools.
10:38
An Overview of Genetic Engineering
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2023
Genetic engineering – the process of purposefully altering an organism’s DNA – has been used to create powerful research tools and model organisms, and has also seen many agricultural applications. However, in order to engineer traits to tackle complex agricultural problems such as stress tolerance, or to realize the promise of gene therapy for treating human diseases, further advances in the field are still needed. Important considerations include the safe and efficient delivery of genetic...
01:30
Alterations in Respiration II
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2024
There are numerous types of normal and abnormal respiration. Based on ventilatory movements, breathing patterns are classified as regular, deep, or shallow. Examples include Biot's breathing, Cheyne-Stokes respiration, Kussmaul's breathing, hyperventilation, and hypoventilation. Each pattern is clinically significant and aids in evaluating patients. In Biot's breathing, the respiratory rate and depth are irregular, alternating between periods of deep gasping and apnea. Common causes include...
01:06
Altered States of Awareness
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2024
Altered states of consciousness represent significant deviations from one's normal mental state. These deviations can range from subtle changes in awareness to profound transformations in perception, thought processes, and sensory experiences. Altered states of consciousness can be triggered by various factors, including drug use, meditation, hypnosis, illness, or even intense fatigue. The ingestion of substances like stimulants or hallucinogens leads to chemical alterations in the brain that...
FAQs
These processes change genetic composition through different routes. Mutation alters DNA, recombination reshuffles genetic material, and DNA damage can generate sequence changes. Because each route changes the genetic information available to cells, studying them helps distinguish naturally arising alterations from those introduced deliberately in experimental biology.
Insertion adds genetic material, deletion removes it, and substitution replaces one nucleotide sequence with another. These distinct changes can affect genes in different ways, altering how cells develop, function, or respond to their environment. Comparing the outcomes of each change helps biologists connect a specific sequence modification with a cellular or organismal trait.
Genetic alteration supports gene-to-trait analysis by allowing researchers to examine what changes when particular genetic information is modified. Observing resulting effects in cells or organisms can clarify links between genes and traits while also helping investigate cellular mechanisms and responses to environmental conditions in biology.
Researchers can introduce targeted changes such as gene insertion, deletion, or substitution to create biological systems that reflect disease-associated genetic conditions. These models provide a way to investigate how altered genes affect cellular function and to explore therapeutic research aimed at understanding or correcting mutations linked with inherited disease.
Controlled genetic changes have applications beyond disease studies. In biotechnology, they can support crop improvement by modifying genetic features of interest, while therapeutic research uses them to investigate disease-associated mutations. The same experimental approach therefore connects basic biological questions with practical efforts to improve organisms or develop disease-focused interventions.
Genome editing is especially relevant when researchers want to modify a specific nucleotide sequence or gene rather than examine genetic change broadly. In therapeutic research, this controlled approach can help investigate disease-associated mutations and support efforts to correct them. Its value lies in connecting a defined DNA change with a disease-related biological outcome.