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Immunology and Infection

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JoVE Journal Immunology and Infection
Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
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Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Article DOI: 10.3791/52533-v • 08:37 min • April 21st, 2015
April 21st, 2015 •
1,2, 1, 1,3,4
1Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, 2Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, 3Department of Genetics, University of North Carolina at Chapel Hill, 4Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill

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Summary
Automatic Translation

Here, we present a protocol to induce colonic inflammation in mice by adoptive transfer of syngeneic CD4+CD45RBhigh T cells into T and B cell deficient recipients. Clinical and histopathological features mimic human inflammatory bowel diseases. This method allows the study of the initiation of colonic inflammation and progression of disease.

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Induction Murine Intestinal Inflammation Adoptive Transfer Effector CD4+CD45RBhigh T Cells Immunodeficient Mice Animal Models Pathogenesis Human Inflammatory Bowel Diseases (IBD) Advantages Disadvantages Experimental Colitis Model Syngeneic Splenic CD4+CD45RBhigh T Cells T And B Cell Deficient Recipient Mice Chronic Intestinal Inflammation Na& 239;ve Effector Cells Disease Onset And Progression Innate Immune Cells Adaptive Immune Cells Regulatory Immune Cells Environmental Exposures Microbiota Methodology Step-by-step Protocol
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