Journal
/
Developmental Biology
/
Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

DOI:
10.3791/52544-v

•

09:31 min

•

April 08, 2015

•

, , , ,
1Immunodynamics,German Rheumatism Research Center, a Leibniz Institute, 2Biophysical Analytics,German Rheumatism Research Center, a Leibniz Institute, 3Max-Delbrück Center for Molecular Medicine, 4Wimasis GmbH, 5Immunodynamics and Intravital Imaging,Charité – University of Medicine

Chapters

  • 00:05Title
  • 01:14Femoral Bone Cryosectioning
  • 02:28Imaging
  • 04:02Random Bone Marrow Positioning Simulation
  • 07:36Results: Representative Analysis of the Bone Marrow Niche Microenvironments for Long Lived Plasma Cells
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

A strategy to quantitatively analyze histological data in the bone marrow is presented. Confocal microscopy of fluorescently labeled cells in tissue sections results in 2-dimensional images, which are automatically analyzed. Co-localization analyses of different cell types are compared to data from simulated images, giving quantitative information about cellular interactions.

Tags

Automated QuantificationCell-cell InteractionsHematopoietic CellsStromal CellsBone MarrowConfocal MicroscopyHistological AnalysisCell RecognitionNeighborhood AnalysisCo-localizationRandom Positioning

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52544?article_type=v

Quantification of Colonic Stem Cell Mutations
check_url/52544?article_type=v

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness
check_url/52544?article_type=v

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
check_url/52544?article_type=v

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
check_url/52544?article_type=v

A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells
check_url/52544?article_type=v

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging
check_url/52544?article_type=v

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
check_url/52544?article_type=v

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
check_url/52544?article_type=v

Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture
check_url/52544?article_type=v

Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture

Read Article