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Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
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Developmental Biology
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JoVE Journal Developmental Biology
Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue

Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue

DOI:
10.3791/52550-v

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09:17 min

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March 04, 2015

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, ,
1Institute of Molecular and Cellular Anatomy,University of Ulm

Chapters

  • 00:05Title
  • 01:17DNA Injection and Electroporation of E15.5 and E18.5 Embryos
  • 03:13Dissection of the Brain
  • 04:21Vibratiome Sectioning and Slice Culture
  • 06:07Fixation of the Sections Followed by Immunofluorescence Staining
  • 07:48Results: Ex Utero Electroporation at E18.5 Following by Organotypic Slice Culture
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol providing a tool to examine regulatory mechanisms of specific genes during hippocampal development. Employing ex utero electroporation and organotypic slice culture allows the up- and down-regulation of the expression of genes of interest in single cells and follow their fate during development.

Tags

Ex Utero ElectroporationOrganotypic Slice CultureMouse GeneticsGene ManipulationCell-autonomous ProcessesProgenitor Cell ProliferationNeuronal DifferentiationIn Utero ElectroporationDentate Gyrus

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