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JoVE Journal
Developmental Biology

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Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
 

Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue

Article DOI: 10.3791/52550
March 4th, 2015

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Summary March 4th, 2015

Here we present a protocol providing a tool to examine regulatory mechanisms of specific genes during hippocampal development. Employing ex utero electroporation and organotypic slice culture allows the up- and down-regulation of the expression of genes of interest in single cells and follow their fate during development.

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