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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
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Biology
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JoVE Journal Biology
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

DOI:
10.3791/52586-v

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10:28 min

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April 14, 2015

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, , , ,
1Diabetes and Islet Biology Group, NHMRC Clinical Trials Centre, Faculty of Medicine,The University of Sydney, 2Biomarkers Laboratory, NHMRC Clinical Trials Centre, Faculty of Medicine,The University of Sydney

Chapters

  • 00:05Title
  • 01:29Probe-based RTqPCR Mature MicroRNA (miRNA) Detection and Microfluidics Array Card Preparation
  • 03:40Microfluidics Card Loading and qPCR
  • 04:59Nanofluidics Array Loading and qPCR
  • 08:05Results: Representative miRNA Profile
  • 09:38Conclusion

Summary

Automatic Translation

Automatic Translation

Circulating microRNAs have recently emerged as promising and novel biomarkers for various cancers and other diseases. The goal of this article is to discuss three different probe-based real-time PCR platforms and methods that are available to quantify and determine the abundance of circulating microRNAs.

Tags

Keywords: Probe-based QPCRMicroRNA QuantificationTranscript AbundanceHigh-throughput PlatformsMicrofluidicsNanofluidics ArraysTaqMan Array CardOpenArraySerumPlasma-EDTA

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