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Chapters
- 00:05Title
- 01:10Mouse Breast Tumor Primary Cell Preparation
- 02:33Lung Metastasis Visualization by Mouse Lung Extraction
- 03:25Mouse Embryonic Fibroblast (MEF) Preparation
- 04:52Results: Representative Primary Tumor Cell and MEF Isolation
- 05:51Conclusion
The goal of this protocol is to study breast tumorigenesis. With this technique, mouse mammary tumors are removed and primary cells are prepared from tumors. A lung extraction protocol is included for studying lung metastasis. Furthermore, another protocol for analyzing mouse embryonic fibroblasts from the mouse embryo is included.
