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LepR<sup> Db</sup> Mouse Model van Type 2 Diabetes: eilandjes van Langerhans Isolatie en live-cell 2-Photon Imaging van intacte Eilandjes
JoVE Journal
Medicine
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JoVE Journal Medicine
Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
DOI:

10:09 min

May 11, 2015

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Chapters

  • 00:05Title
  • 01:31Reagent Preparation
  • 03:22Islet Isolation and Islet Culturing
  • 06:50Live Cell Photon Imaging
  • 08:06Results: Pancreatic Islet Yield and Insulin Secretion Using 2-Photon Assay
  • 09:37Conclusion

Summary

Automatic Translation

We present here a protocol for the isolation of islets from the mouse model of type 2 diabetes, Leprdb and details of a live-cell assay for measurement of insulin secretion from intact islets that utilizes 2 photon microscopy.

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