Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

The overall goal of this procedure is to measure changes in membrane properties of olfactory sensory neurons in response to odor stimulants. This is accomplished by first dissecting the olfactory epithelium and transferring it into a perfused chamber. The second step is to locate and choose an OSN of interest under the microscope.

The dendritic knob of the chosen OSN is then patch clamped and analyzed while being stimulated with odorants. Ultimately, perforated patch clamping of OSN dendritic knobs characterizes OSN functional properties at the membrane level. This method can help address key questions in the olfaction fields, such as recording olfactory, sensory neurons, expressing defined odor art receptors, And also patch clamp recordings.