Name: Therapeutic Effectiveness of a Dietary Supplement for Management of Halitosis in Dogs (Video) | JoVE Journal
Uploaded: 2015-07-06T14:00:00.000+00:00
Duration: 7 min 33 s
Description: Learn to manage halitosis in dogs using a dietary supplement and portable chromatograph for measuring volatile sulfur compounds. This method enables rapid assessment of treatment effectiveness in veterinary practice.

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5 minutes

This review was not supported by grants. We thankSanypet S.p.a. (Padua, Italy) for kindly providing the dietary supplement and placebo used in this study. We also thank San Patrignano Community which kindly participated in the study by supplying all dogs.

This clinical evaluation was planned accordingly with the European legislation (86/609/EU) and the experimental protocol was approved by the Ethics and Welfare Animal Committee of the AIRMO Research Center (Milan, Italy).
1. Dogs and Food Selection
<ol>
	<li>Randomly divide 32 dogs of different breeds (7.01 &#177; 0.20 year [mean &#177; standard error of mean (SEM)]; 34.05 &#177; 1.47 Kg [mean &#177; SEM]; 12 males and 20 females) suffering from chronic halitosis into 2 groups, providing them with either the dietary supplement or the placebo over a period of 30 days.</li>
	<li>Ensure that each dog receives the appropriate dose of dietary supplement or placebo based on the animal&#8217;s weight in kilograms, according to the manufacturer&#8217;s instructions.&#160;(Table 1).</li>
	<li>After a 30 day treatment, ensure that both groups have a 10 day wash out, where all animals are fed with placebo. &#160;At the end of the wash out period, the feeding regimen for the two groups are reversed such that the control group receives the dietary supplement for another 30 days and the treatment group receives the placebo.</li>
	<li>Two veterinary inspections performed on the dogs in both groups, one before and one after the 30 day treatment.</li>
</ol>
2. The Gas Chromatograph
Note: The gas chromatograph is a portable device which measures the volatile sulphur compounds (VSC), hydrogen sulfide, methyl mercaptan, and dimethyl sulfide, which are the major causative factors of halitosis, providing information about the concentration of each gas measured. Measuring each VSC helps to identify the cause of halitosis and assess treatment efficacy.
<ol>
	<li>Using the procedure outlined in sections 3 and 4, examine volatile sulphur compounds (VSC) before treatment with the dietary supplement (time 0), 10 days post-treatment (time 10), 20 days post-treatment (time 20) and at the end of treatment (time 30).</li>
</ol>
3. Gas Chromatograph Setting
<ol>
	<li>Turn on the main switch at the back of the unit. Note: The initial screen displays the word &#8220;MEASUREMENT MODE&#8221;.</li>
	<li>Press the ENTER key to select MEASUREMENT MODE. 
	Note: WAIT is displayed on the screen and the standby time required to stabilize the measuring device is displayed next to the word &#8220;COUNTER&#8221;. When the standby time has come to an end, the device is automatically ready to measure and the READY indicator lamp turns on.</li>
</ol>
4. Dogs Breath Sampling and Analysis
<ol>
	<li>Run the Data Manager software.</li>
	<li>Click &#8220;Measurement&#8221; after confirming that &#8220;Current status&#8221; is &#8220;Ready to measure&#8221;</li>
	<li>Enter ID and click &#8220;Select&#8221;. Note: A message is displayed at the upper right-hand corner of the window.
	<ol>
		<li>If the message displayed is &#8220;Inject oral gas&#8221;, start the measurement.</li>
		<li>If the message displayed is &#8220;The device is being ready&#8221;, then wait.</li>
	</ol>
	</li>
	<li>By means of a 1&#160;ml disposable syringe, collect the breath from the dog&#8217;s mouth by placing the syringe in the dog&#8217;s labial commissure, slowly pulling the plunger and removing the syringe from the dog&#8217;s mouth .</li>
	<li>Adjust the volume of the syringe to 1 ml.</li>
	<li>Attach the supplied needle to the end of the syringe and, after opening the lid, eject the sample into the inlet on the device main unit by pushing the plunger. 
	Note: Measurements start automatically and the READY indicator lamp goes off. Measurement is completed in 8 min and the result is automatically displayed (display can be changed to &#8220;Chromatogram&#8221;). The Judgment window appears upon completing the measurement. Measurement history and measured data can be displayed .</li>
	<li>Push ENTER to store the results into the unit. 
	Note: Data are expressed in standard units of parts per billion (ppb) and/or ng/10 ml.</li>
	<li>Then wait 1 min to stabilize the device and perform a new measurement. Note: At the end of this time, the lamp turns on meaning that the device is ready.</li>
</ol>

The authors have nothing to disclose.

Halitosis is a common problem in dogs, representing a psychosocial issue significantly affecting the pet-owner relationship19. Bad breath comes from microbial metabolism of both exogenous and endogenous protein substrates within the oral cavity20 which results in volatile sulphur compound production, and is worsened by other factors including acute necrotising ulcerative gingivitis, infected extraction site, debris under dental appliances, ulcers, tonsilloliths and low hygiene21-23. The main agents responsible for bad breath production are Gram-negative bacteria24 and their increase is related to a thickening of dental plaque25. Good oral hygiene along with specific diets can reduce the microbial load to the physiological level, reducing halitosis and avoiding gengivitis and periodontal diseases that have unpleasant effects on breath26.
This view is supported by our study showing that, after 10 days of dietary supplement intake, an overall improvement was observed in 29 out of 32 dogs (90%) presenting a moderateto-severe malodor and, at the end of the evaluation, 19 out of 29 dogs (65%) who achieved an improvement acquired a stabile physiological condition regarding VSCs concentration.
Our investigation broaches the very intriguing issue of the possible administration of a dietary supplement to dogs with breath malodor by basically introducing fish hydrolized proteins, sage, Ribes nigrum L., thyme, lysozyme, propolis, bioflavonoids and vitamin C in the daily nutritional schedule. These compounds, widely used in traditional medicine, have been already proven effective both in vitro and in vivo.
Specifically, fish proteins were chosen on the basis of previous chromatographic studies which pointed out the presence of great amounts of hydrogen sulfide, methanethiol, ethanethiol, dimethyl sulfide, and ethylene sulfide in beef proteins27. Literature studies have also shown an antimicrobial activity of sage and Ribes nigrum L. against Streptoccocus mutans which is known to be one of the oral pathogens (along with Porphyromonas gingivalis and Candida albicans) responsible for oral malodor and dental caries formation28-30. Bioflavonoids and vitamin C have been recognized as both antiinflammatory and bacteriostatic agents limiting the growth of certain bacteria associated with periodontal diseases31.
Regarding propolis and thyme, a reduction in malodor production from incubated whole saliva was demonstrated by Sterer et al.32 The bacteriostatic activity of propolis has been widely demontrated in literature, ranging from limiting the quantity of bacterial plaque33-37 to decreasing the tolerance of microorganisms to acid pH38, showing greater effectiveness than chlorhexidine gluconate solutions39. Moreover, propolis was proven effective in reducing teeth caries in rats limiting the number of microorganisms, slowing down synthesis of insoluble glucans, and glucosyltransferase activity40.
Our study presents some limitations such as the sensitivity of the instrument, if compared with other available techniques (e.g. bumpy WO3 hemitube nanostructure assisted by O2 plasma surface modification with functionalization of graphene-based material41, electrospun SnO2 nanofibers sensitized with reduced graphene oxide nanosheets42, benzoyl-DL-arginine-naphthylamide (BANA) test43, High Performance Liquid Chromatography (HPLC)44 and Solid-Phase Micro Extraction-Gas Chromatography/Mass Spectrometry (SPME-GC/MS45). On the other hand, the device employed in this study might allow, for instance, fast data collection to rapidly achieve an early diagnosis of viral hepatitis B, which is also characterized by elevated dimethile sulfide levels46 or to establish a state of systemic and/or respiratory disease (such as pneumonia, pulmonary emphysema and bronchitis) that are characterized by elevated hydrogen sulfide, methyl mercaptan and dimethyl sulfide levels47.
During the procedure we realized that the time available to collect the breath from a dog&#8217;s mouth was slightly lower than that required (few seconds against 30 sec suggested by the manufacturer). This problem was successfully overcome by repeating each measurement three times. A critical step during the experiment was breath collection. In order not to leave the dog with his mouth open for too long without damaging the syringe, we placed the syringe in the dog&#8217;s labial commissure in order to safely collect the breath.
As observed in humans, further applications of this technique will be the prevention of breath-related diseases, i.e. systemic and respiratory, in dogs and more generally in pets. In summary, our study showed that the dietary supplement was effective in reducing chronic halitosis in dogs. Our results might be also in agreement with what is proposed by Porter et al., who hypothesized a possible interplay between halitosis and the gastrointestinal tract suggesting that a therapy for halitosis with oral origin might also have some effect upon gut bacteria composition which is known to be responsible, in some cases, for volatile sulphur compounds production48.&#160;Nevertheless, a dysbiosis might also occur after a massive use of antibiotics49, or meat derived from intensive livestocks and commonly used in most of animal and human food50,51.&#160;

Oral malodor, also known by the term halitosis, is a common complaint which causes embarrassment in interpersonal social relationships1 as much as body malodor2. Although most epidemiological data on oral malodor is limited in accuracy and sensitivity due to a subjective self-estimation, studies report that 30-50% of the population is affected by this pathology3-6. Some studies have also evidenced that it is more prevalent in men than in women regardless of age with a ratio 3:1 and the ratio is slightly higher in people over 20 years7.
Halitosis can be staged depending on its etiology: type 1 (oral), type 2 (airway), type 3 (gastroesophageal), type 4 (blood-borne) and type 5 (subjective); however, it has been hypothesized that halitosis could be potentially considered the sum of these types in any combination, superimposed on the physiologic odour present in health (Type 0)8,9. Scully et al., who widely described and characterized aetiopathogenesis and management of halitosis, reported that in some patients it has an extra-oral aetiology and, in a few of them, metabolic anomalies are involved10. The authors pointed out that volatile sulphur compounds (VSC) and some predisposing factors, e.g. poor oral hygiene, hyposalivation, dental appliances, gingival and periodontal disease and mucosal disease, are mainly responsible for the malodor. Further, they proposed oral health improvement, anti-malodor therapies and malodor counteractives as a basic conventional approach. Moreover, they also proposed the use of probiotics and vaccines against malodor causing bacteria.
Human and animal companions, i.e. cats and dogs, can be affected by food-related adverse reactions which can involve several systems such as the gastroenteric, cutaneous, otological, ocular, urinary and respiratory systems11-13. However, several animal studies have pointed out that the nasal chamber is one of most involved systems where tartar14, stomatitis15, and halitosis16 are quite often present. Furthermore, bacteria that cause halitosis can be transmitted from animal companions to humans17. Iwanicka-Grzegorek quantified sulphur compounds and low molecular mass amines in saliva from 84 patients suffering from halitosis and 40 healthy controls. More than 80% of patients belonging to the halitosis group reported having pets in childhood (P &#60;0.001) and over 70% still owned a pet (P &#60;0.001).
Therefore nutrition can be used to manage halitosis. Moreover, nutrition plays a pivotal role in tooth development, oral and gum tissue integrity, bone resistance and even dental and oral disease management18. Based on such considerations, at the Italian Association for Oncological Research Disease (AIRMO) Center, we run&#160;a randomized placebo-controlled crossover clinical evaluation involving 32 dogs suffering from halitosis in order&#160;to investigate the efficacy of a dietary supplement vs. a commercially available pet food.

<table><tbody><tr><td>OralChroma CHM-1</td><td>FIS Inc.</td><td>10061285</td><td>portable gas cromatograph</td></tr><tr><td>FORZA10 Oral Active</td><td>SANYpet .Sp.a.</td><td></td><td>dietary supplement</td></tr><tr><td>InJ/Light-100 PCS</td><td>Rays S.p.a.</td><td>INJ5171112</td><td>disposable syringes&amp;nbsp;</td></tr></tbody></table>

therapeutic effectiveness of a dietary supplement for management of halitosis in dogs

Therapeutic Effectiveness of a Dietary Supplement for Management of Halitosis in Dogs

Halitosis is a common complaint involving social and communicational problems in humans and also affects the pet-owner relationship. In this randomized placebo-controlled crossover clinical evaluation, we assessed&#160;the effectiveness of a dedicated dietary supplement to improve chronic halitosis in 32 dogs of different breeds and ages. This protocol describes how to evalute the presence of oral volatile suphur compunds, e.g. methyl mercaptan, hydrogen sulfide and dimethyl sulfide, by means of a portable gas chromatograph device coupled with a syringe, which was used to collect the breath, and a dedicated software, which allows the operator to monitor each compound concentration during each measurement, in a relatively short time (8 min).
A significant modification of halitosis parameters was observed after 30 days since the beginning of treatment (p &#60;0.05), while a long-lasting effect was still observed even 20 days after the suspension of the treatment. Portable gas chromatograph, which is also widely used in clinical practice, can be therefore used to confirm&#160;and control halitosis in humans and animals. Even though human and animal species present some differences,&#160;this innovative and alternative therapy for halitosis management might be extended to human clinical practice as an adjuvant dietary approach.

Both placebo and dietary supplement were adjusted in order to provide similar nutritional and caloric intake and to satisfy the nutritional requirement of adult dogs, in particular. Their analytical composition was: crude protein 26%, crude oils and fats 13%, crude fiber 2.5%, crude ash 8% and Metabolized Energy (EM) 3464 kcal/kg. Metabolized energy was calculated according to the Nutritional Guidelines for complete and complementary pet food for cats and dogs by The European Pet Food Industry Federation. Placebo was composed of dehydrated chicken meat, rice, animal fat, dehydrated eggs, oat, dry beet pulp, flax seeds, hydrolysed animal proteins, fish oil, vegetable oil, calcium carbonate, dry yeast beer, monocalcium phosphate, sodium chloride. Dietary supplement differed from the placebo for the presence of active substances contained in special heart-shaped cold pressed tablets (European patent n. EP 2526781) included in the complete food in a percentage of 6-7%, and for the protein source, fish meal instead chicken meal. Tablets contained propolis (0.0161%), Salvia officinalis (0.0087%), egg albumen (lysozyme 0.0078%), dehydrated orange extract (bioflavonoids 0.0077%), Thymus vulgaris (0.0127%), Ribes nigrum (0.0040%).
Data were analyzed, presented as the means ± standard error of the mean and checked for normality using the D’Agostino-Pearson normality test. A multiple t-test was used to compare changes in VSCs score at follow-up versus baseline for each treatment modality.
Figure 1A shows a significant decrease in hydrogen sulfide concentration in treated animals, from a baseline value of 0.43 ng/ml to 0.06 ng/ml, after 30 days treatment. Figure 1B shows a significant decrease of methyl mercaptan concentration in treated animals, from a baseline value of 0.30 ng/ml to 0.06 ng/ml, after 30 days treatment. Figure 1C shows a significant decrease in dimethyl sulfide concentration in treated animals, from a baseline value of 0.28 ng/ml to 0.13 ng/ml, after 30 days treatment.
Figure 2A shows a significant decrease in hydrogen sulfide concentration, from a baseline value of 0.37 ng/ml to 0.10 ng/ml, after 20 days treatment. In Figure 2B a similar trend is observed also for methyl mercaptan which shows a significant decrease of the compound concentration, from a baseline value of 0.24 ng/ml to 0.05 ng/ml, after 30 days treatment. As to dimethyl sulfide, Figure 2C, a significant decrease of the compound concentration, from a baseline value of 0.32 ng/ml to 0.11 ng/ml, was observed after 30 days treatment.
<table border="0" cellpadding="0" cellspacing="0">
	<tbody>
		<tr>
			<td>BODY WEIGHT (Kg)</td>
			<td>DIETARY SUPPLEMENT AMOUNT PER DAY (g)</td>
		</tr>
		<tr>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>1 - 10</td>
			<td>30 - 180</td>
		</tr>
		<tr>
			<td>11 - 20</td>
			<td>190 - 300</td>
		</tr>
		<tr>
			<td>21 - 35</td>
			<td>310 - 455</td>
		</tr>
		<tr>
			<td>36 - 50</td>
			<td>465 - 595</td>
		</tr>
	</tbody>
</table>
Table 1: Daily Amount of Food Provided to Dogs.
<img alt="Bar graph of H2S, CH3SH, (CH3)2S concentrations; before/after treatment comparison; control/treatment groups." src="/files/ftp_upload/52717/52717fig1.jpg" data-altupdatedat="1787630408000" data-alt="Figure 1"> 
Figure 1: (A) Graphical Representation of Hydrogen Sulfide Concentration Trend in Treated (n = 16) vs Control (n = 16) Group During the 30 Days Treatment Period; *p &lt;0.05; (B) Graphical Representation of Methyl Mercaptan Concentration Trend in Treated (n = 16) vs Control (n = 16) Group During the 30 Days Treatment Period; *p &lt;0.05; (C) Graphical Representation of the Dimethyl Sulfide Concentration Trend in Treated (n = 16) vs Control (n = 16) Group During the 30 Days Treatment Period; *p &lt;0.05.
<img alt="Bar graphs comparing control vs treated concentration of H2S, CH3SH, (CH3)2S after washout." src="/files/ftp_upload/52717/52717fig2.jpg" data-altupdatedat="1787630408000" data-alt="Figure 2"> 
Figure 2: (A) Graphical Representation of Hydrogen Sulfide Concentration Trends in Treated (n = 16) vs Control (n = 16) Group after the 10 Days Wash Out Period; *p &lt;0.05; (B) Graphical Representation of Methyl Mercaptan Concentration Trend in Treated (n = 16) v.s. Control (n = 16) Group after the 10 Days Wash Out Period; *p &lt;0.05; (C) Graphical Representation of Dimethyl Sulfide Concentration Trends in Treated (n = 16) v.s. Control (n = 16) Group after the 10 Days Wash Out Period; *p &lt;0.05.

We describe a simple approach for diagnosis of halitosis in dogs as well as a dietary approach for its management. This protocol may be extended to the management of halitosis in humans in the near future.

Halitosis is a common complaint involving social and communicational problems in humans and also affects the pet-owner relationship. In this randomized ...

The overall goal of this procedure is to demonstrate the modulating activity of a specific diet for halitosis management in dogs by means of a portable chromatograph for human use. This is accomplished by first randomly dividing dogs of different breeds, suffering from chronic halitosis into two groups. A baseline measurement of the volatile sulfur compounds or VSC, which are the major causative factors of halitosis, is then acquired from the dogs using the oral chroma gas chromatograph.Then a specific diet is provided to the dog's belonging to the treatment group for a 30 day period while the other group receives a placebo measurement of the halitosis related compounds is repeated throughout the treatment period and at the end of treatment. The final step is to allow both groups to have a washout period where all animals are fed with placebo. At the end of the washout period, the two groups are inverted, such that the control group receives the dietary supplement and the treatment group receives the placebo.The main advantage of this technique over existing methods like high performance liquid chromatography and solid phase macro extraction gas chromatography, mass spectrometry, is that is portable rapid and does not require a long training to perform an independent analysis. Demonstrating the procedure will be Francesca, a graduate student from my laboratory. To begin randomly divide 32 dogs of different breeds, suffering from chronic halitosis into two groups, subject the dogs to a veterinary inspection.The oral chroma gas chromatograph is a portable device which measures the volatile sulfur compounds or VSC, which are the major causative factors of halitosis. Before treatment with a dietary supplement measure the dog's level of VSC to do so. First turn on the main switch at the back of the unit.The initial screen displays the words measurement mode. Then press the enter key to select measurement mode. Wait is displayed on the screen and the standby time required to stabilize the measuring device is displayed next to the word counter.When the standby time has come to an end, the device is automatically ready to measure and the ready indicator lamp turns on. Next, run the data manager software. Click measure.Enter the ID and click okay. A message is displayed that says, this ID was not found in the data folder. Would you like to register this ID newly at this point, click yes.Then type, name any comment, sex and date of birth of the subject, and afterwards, click Okay. A message is displayed that says, click okay to start receiving now click okay on the displayed message. Next, use a one milliliter disposable syringe to collect the breath from the dog's mouth by placing the syringe in the dog's labial commissure, slowly pulling the plunger and removing the syringe from the dog's mouth.Adjust the volume of the syringe to one cubic centimeter. Attach the supplied needle to the end of the syringe, and after opening the lid, eject the sample into the inlet on the device main unit by pushing the plunger measurements start automatically and the ready indicator lamp goes off. Measurement is completed in eight minutes and the result is automatically displayed.The judgment window appears upon completing the measurement, measurement history and measure data can be displayed. Push enter to store the results into the unit. Then wait one minute to stabilize the device and perform a new measurement.At the end of this time, the lamp turns on to indicate that the device is ready. After the baseline measurements have been taken for each dog, provide one group with a dietary supplement and one with a placebo over a period of 30 days. See the text protocol for the correct dose of the dietary supplement or placebo.Repeat the VSC measurement at 10 days post-treatment, 20 days post-treatment, and at the end of treatment after a 30 day treatment, ensure that both groups have a 10 day washout where all animals are fed with placebo. At the end of the washout period. Invert the two groups such that the control group receives the dietary supplement for another 30 days and the treatment group receives the placebo.After repeating the VSC measurement a final time, subject the dogs to another veterinary inspection, a significant decrease in hydrogen sulfide concentration is observed in treated animals from a baseline value of 0.43 nanograms per milliliter to 0.06 nanograms per milliliter after 30 days of treatment. As shown here, there is a significant decrease of methylome captan concentration in treated animals from a baseline value of 0.30 nanograms per milliliter to 0.06 nanograms per milliliter. After 30 days of treatment, a significant decrease in dimethyl sulfide concentration was also observed in treated animals from a baseline value of 0.28 nanograms per milliliter to 0.13 nanograms per milliliter After 30 days of treatment, the results are comparable.After the 10 day washout period, there was a significant decrease in hydrogen sulfide concentration after 20 days of treatment from a baseline value of 0.37 nanograms per milliliter to 0.10 nanograms per milliliter. A similar trend is observed for methylome captan, which shows a significant decrease of the compound concentration from a baseline value of 0.24 nanograms per milliliter to 0.05 nanograms per milliliter after 30 days of treatment. As to dimethyl sulfide a significant decrease of the compound concentration from a baseline value of 0.32 nanograms per milliliter to 0.11 nanograms per milliliter was observed after 30 days of treatment.After watching this video, you should have a good understanding of how to easily monitor halitosis compounds variation following a specific diet While attempting this procedure, it is important to remember to leave the device to stabilize for eight minutes before each measurement.

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Learn to manage halitosis in dogs using a dietary supplement and portable chromatograph for measuring volatile sulfur compounds. This method enables rapid assessment of treatment effectiveness in veterinary practice.

Therapeutic Effectiveness of a Dietary Supplement for Management of Halitosis in Dogs

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