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Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans
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Chapters
- 00:05Title
- 01:00Instruments, Culture Media and Dishes
- 01:58Preparation of Agarose Microchambers
- 04:50Agarose Microfluidic Chambers Imaging
- 05:37Calcium Imaging
- 06:27Results: Long-term Calcium Imaging
- 07:20Conclusion
Imaging behavior and neural activity over long time scales without immobilization of the animal is a prerequisite to understand behavior. Agarose microfluidic chambers imaging (AMI) can be used to image neural activity and behavior for all life stages of Caenorhabditis elegans.
