Journal
/
Biology
/
Comet Assay as an Indirect Measure of Systemic Oxidative Stress
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Comet Assay as an Indirect Measure of Systemic Oxidative Stress

Comet Assay as an Indirect Measure of Systemic Oxidative Stress

DOI:
10.3791/52763-v

•

08:23 min

•

May 22, 2015

•

, , , ,
1Department of Ophthalmology,University of Basel, 2Department of Biomedicine,University of Basel

Chapters

  • 00:05Title
  • 01:31Isolation of Leukocytes
  • 03:44Lysis and Electrophoresis
  • 06:14Results: The Comet Assay Measures of Oxidative Stress
  • 07:47Conclusion

Summary

Automatic Translation

Automatic Translation

Comet assay measures DNA breaks, induced by different factors. If all factors (except oxidative stress) causing DNA damage are kept constant, the amount of DNA damage is a good indirect parameter of oxidative stress. The goal of this protocol is to use comet assay for indirect measurement of oxidative stress.

Tags

Keywords: Comet AssayOxidative StressReactive Oxidative Species (ROS)DNA DamageCell Gel ElectrophoresisDNA BreaksDNA MigrationElectrophoresis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52763?article_type=v

A Behavioral Assay to Measure Responsiveness of Zebrafish to Changes in Light Intensities
check_url/52763?article_type=v

Application of an In vitro DNA Protection Assay to Visualize Stress Mediation Properties of the Dps Protein
check_url/52763?article_type=v

Analysis of Oxidative Stress in Zebrafish Embryos
check_url/52763?article_type=v

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro
check_url/52763?article_type=v

Measuring Oxidative Stress Resistance of Caenorhabditis elegans in 96-well Microtiter Plates
check_url/52763?article_type=v

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
check_url/52763?article_type=v

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity
check_url/52763?article_type=v

In Vivo Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver
check_url/52763?article_type=v

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
check_url/52763?article_type=v

A Plate Competition Assay As a Quick Preliminary Assessment of Disease Suppression

Read Article