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Journal / Bioengineering / Hybrid µCT-FMT imaging and image analysis
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Bioengineering
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JoVE Journal Bioengineering
Hybrid µCT-FMT imaging and image analysis
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Hybrid µCT-FMT imaging and image analysis

Article DOI: 10.3791/52770-v • 13:45 min • June 4th, 2015
June 4th, 2015 •
*1, *1, 1, 2, 2, 1,3, 1, 1
1Experimental Molecular Imaging, RWTH Aachen University, 2Institute for Biomedical Engineering - Biointerface Laboratory, RWTH Aachen University, 3Utrecht Institute for Pharmaceutical Sciences, Utrecht University
* These authors contributed equally

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Summary
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We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.

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Hybrid Imaging µCT-FMT Imaging Fluorescence-mediated Tomography Image Analysis Biodistribution Assessment Disease Progression Molecular Probes Reporter Genes Anatomical Modality Micro Computed Tomography Image Processing Quantitative Measurements Multimodal Data Sets Registration Fluorescence Reconstruction Mouse Shape Consideration Organ Segmentations Interactive Segmentation Tool Biodistribution Curves
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