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Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy
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Chapters
- 00:05Title
- 01:22Dissecting Cerebellums from P10 Rats
- 02:26Preparing Acute Cerebellar Slices
- 04:27Fluorescent Staining of Living Interneurons
- 05:44Ex Vivo Imaging
- 06:27Cell Tracking Using ImageJ
- 07:25Results: Analysis of Granule Cell Migration
- 08:26Conclusion
During postnatal cerebellum development, immature granule cells originating from the germinal zone exhibit distinct modalities of migration to reach their final destination and to establish neuronal networks. This protocol describes the preparation of cerebellar slices and the confocal macroscopic approach used to investigate the factors that regulate neuronal migration.
