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Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys<sub>2</sub>-His<sub>2</sub> Zinc-finger Domains
JoVE Journal
Biology
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JoVE Journal Biology
Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains

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11:24 min

March 25, 2015

DOI:

11:24 min
March 25, 2015

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Summary

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Zinc-finger domains are intrinsically cell-permeable and capable of mediating protein delivery into a broad range of mammalian cell types. Here, a detailed step-by-step protocol for implementing zinc-finger technology for intracellular protein delivery is presented.

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