Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys<sub>2</sub>-His<sub>2</sub> Zinc-finger Domains

Thomas Gaj; Jia Liu

doi:10.3791/52814

Journal

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Biology

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Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys₂-His₂ Zinc-finger Domains

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys<sub>2</sub>-His<sub>2</sub> Zinc-finger Domains

JoVE Journal
Biology

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JoVE Journal Biology

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys₂-His₂ Zinc-finger Domains

9,857 Views

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11:24 min

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March 25, 2015

DOI:

10.3791/52814-v

DOI:

10.3791/52814-v

•

11:24 min

March 25, 2015

•

1 Views

Thomas Gaj*¹, Jia Liu*^1,2

¹Departments of Chemistry and Cell and Molecular Biology,The Scripps Research Institute, ²Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University

Summary

Zinc-finger domains are intrinsically cell-permeable and capable of mediating protein delivery into a broad range of mammalian cell types. Here, a detailed step-by-step protocol for implementing zinc-finger technology for intracellular protein delivery is presented.