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A New Application of the Electrical Penetration Graph (EPG) for Acquiring and Measuring Electrical Signals in Phloem Sieve Elements
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A New Application of the Electrical Penetration Graph (EPG) for Acquiring and Measuring Electrical Signals in Phloem Sieve Elements

A New Application of the Electrical Penetration Graph (EPG) for Acquiring and Measuring Electrical Signals in Phloem Sieve Elements

DOI:
10.3791/52826-v

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14:16 min

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July 02, 2015

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1Department of Plant Physiology and Biophysics,University of Würzburg, 2EPG Systems, Wageningen, The Netherlands

Chapters

  • 00:05Title
  • 03:18Aphid Rearing
  • 04:14Making an Insect Electrode
  • 05:58Wiring an Insect for Electrical Penetration Graph (EPG) Recordings
  • 08:27EPG Recording
  • 11:27Results: Electrical Response to Wounding in Central and Marginal Veins in Leaves of Arabidopsis thaliana
  • 13:18Conclusion

Summary

Automatic Translation

Automatic Translation

Electrical Penetration Graph (EPG) is a well-established technique for studying the feeding behavior of stylet-bearing insects. Here we show a new application of EPG as a non-invasive tool for the acquisition of intracellular electrophysiology recordings of sieve elements (SEs), the cells that form the phloem vasculature in plants.

Tags

Keywords: Electrical Penetration GraphEPGPhloem Sieve ElementsIntracellular ElectrophysiologyPlant Electrical SignalsWound-induced SignalsArabidopsis ThalianaPhloem-feeding InsectsPlant VasculatureIn Vivo Electrophysiology

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