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JoVE Journal
Immunology and Infection

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Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
 

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Article doi: 10.3791/52830
June 5th, 2015

Summary June 5th, 2015

Synthesis of human monoclonal antibodies is the first step in studies aimed at unraveling the pathophysiological mechanisms of auto-antibody-mediated immune responses. We have developed a protocol to generate recombinant human immunoglobulin G (IgG) monoclonal antibodies from blood sorted B cells, including B-cell isolation, antibody cloning and in vitro synthesis.

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