18,294 Views
•
10:32 min
•
June 05, 2015
DOI:
Summary
Automatically generated
Synthesis of human monoclonal antibodies is the first step in studies aimed at unraveling the pathophysiological mechanisms of auto-antibody-mediated immune responses. We have developed a protocol to generate recombinant human immunoglobulin G (IgG) monoclonal antibodies from blood sorted B cells, including B-cell isolation, antibody cloning and in vitro synthesis.
Related Videos
11:31
Generation of Recombinant Influenza Virus from Plasmid DNA
Related Videos
30195 Views
09:19
Recombinant Retroviral Production and Infection of B Cells
Related Videos
14049 Views
14:23
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
Related Videos
23933 Views
10:03
Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells
Related Videos
16809 Views
09:47
Generation of Human Alloantigen-specific T Cells from Peripheral Blood
Related Videos
12881 Views
08:09
Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
Related Videos
15727 Views
09:42
Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Related Videos
40922 Views
07:35
Generation of Human Monocyte-derived Dendritic Cells from Whole Blood
Related Videos
20092 Views
07:55
Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
Related Videos
11585 Views
13:14
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
Related Videos
10285 Views
Read Article
Cite this Article
Nogales-Gadea, G., Saxena, A., Hoffmann, C., Hounjet, J., Coenen, D., Molenaar, P., Losen, M., Martinez-Martinez, P. Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells. J. Vis. Exp. (100), e52830, doi:10.3791/52830 (2015).
Copy