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Journal / Immunology and Infection / Anwendung von Langzeit kultiviert Interferon-γ…
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Anwendung von Langzeit kultiviert Interferon-γ Enzyme-linked Immunospot Assay zur Beurteilung Effektor und Gedächtnis-T-Zell-Antworten in Cattle
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Anwendung von Langzeit kultiviert Interferon-γ Enzyme-linked Immunospot Assay zur Beurteilung Effektor und Gedächtnis-T-Zell-Antworten in Cattle

Article DOI: 10.3791/52833-v • 15:58 min • July 11th, 2015
July 11th, 2015 •
1,2, 1, 3, 3, 4, 1, 1
1Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 2Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, 3UK Veterinary Laboratories Agency, 4Visual Services, National Centers for Animal Health, Animal and Plant Health Inspection Service, United States Department of Agriculture

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Langzeit-kultivierten Interferon-γ enzymgebundenen immunospot Assay wird als Maß der zentralen Speicher-Antworten verwendet, und korreliert mit Schutz antimykobakteriellen Impfstoff Antworten. Mit diesem Test werden die peripheren mononukleären Blutzellen mit mycobakteriellen Antigene und IL-2 für 14 Tage, so dass die Differenzierung und Expansion des zentralen Gedächtnis-T-Zellen stimuliert.

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Immunologie Ausgabe 101, Rindertuberkulose CD4-T-Zellen Impfstoff.
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