Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel

The overall goal of this procedure is to monitor the formation of an induced thrombosis in an exposed mouse vessel. This is accomplished by first exposing the vessel of interest. Next, a small piece of filter paper soaked with ferric chloride is deposited on the vessel to induce the thrombus on the mesenteric vessel.

The aggregation of the thrombus forming cells are monitored by in vial microscopy on the carotid artery. The changes in the blood flow induced by the thrombus is measured with a doppler flow meter. The use of chloride to induce a thrombosis in the CED artery of reds has been first established in 1990 by Professor Coz and colleagues.