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JoVE Journal
Immunology and Infection

This content is Open Access.

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants
 

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants

Article doi: 10.3791/52851
May 13th, 2015 Usage Statistics

Summary May 13th, 2015

Coxiella burnetii is an obligate intracellular Gram-negative bacterium responsible for the zoonotic disease Q fever. Here we describe methods for the generation of Coxiella fluorescent transposon mutants as well as the automated identification and analysis of the resulting internalization, replication and cytotoxic phenotypes.

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