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A Method for Remotely Silencing Neural Activity in Rodents During Discrete Phases of Learning
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JoVE Journal Behavior
A Method for Remotely Silencing Neural Activity in Rodents During Discrete Phases of Learning

A Method for Remotely Silencing Neural Activity in Rodents During Discrete Phases of Learning

DOI:
10.3791/52859-v

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09:22 min

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June 22, 2015

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,
1Department of Neuroscience,Oberlin College

Chapters

  • 00:05Title
  • 01:15Stereotaxic Surgery for Viral Infusion
  • 04:43Pharmacological Treatment (Administration of the Designer Drug) and Behavioral Procedure (Sensory Preconditioning)
  • 07:26Post Procedure Analyses
  • 07:54Results: Sensory Preconditioning in Silenced and Control Rats
  • 08:53Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to temporarily and remotely silence neuronal activity in discrete brain regions while rats are engaged in learning and memory tasks. The approach combines pharmacogenetics (Designer-Receptors-Exclusively-Activated-by-Designer-Drugs) with a behavioral paradigm (sensory preconditioning) that is designed to distinguish between different components of learning.

Tags

Keywords: PharmacogeneticsDesigner-Receptors-Exclusively-Activated-by-Designer-DrugsSensory PreconditioningViral-mediated DeliveryInhibitory G-protein Coupled ReceptorNeuronal Activity SilencingLearning And MemoryBehavioral ParadigmBrain Region TargetingDose-dependent Effects

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