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Immunology and Infection

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Co-immunoprecipitation of the Mouse Mx1 Protein with the Influenza A Virus Nucleoprotein

Article DOI: 10.3791/52871 • 09:39 min
April 21st, 2015 •
1,2, 1,2, 1,2
1Inflammation Research Center, VIB, 2Department of Biomedical Molecular Biology, Ghent University

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April 21st, 2015

This co-immunoprecipitation protocol allows to study the interaction between the influenza A virus nucleoprotein and the antiviral Mx1 protein in human cells. The protocol emphasizes the importance of N-ethylmaleimide for successful co-immunoprecipitation of Mx1 and influenza A virus nucleoprotein.

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