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Journal / Developmental Biology / Generation af induceret pluripotente stamceller…
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Developmental Biology

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JoVE Journal Developmental Biology
Generation af induceret pluripotente stamceller fra Frozen Buffy Coats hjælp Ikke integrere episomale plasmider
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Generation af induceret pluripotente stamceller fra Frozen Buffy Coats hjælp Ikke integrere episomale plasmider

Article DOI: 10.3791/52885-v • 10:52 min • June 5th, 2015
June 5th, 2015 •
*1, *1, 1, 1, 2, 2, 3, 1, 1, 1
1Center for Biomedicine, European Academy Bozen/Bolzano (EURAC), 2Laboratory of Medical Genetics, Fondazione IRCCS Ca´ Granda, Ospedale Maggiore Policlinico, 3Del E. Webb Center for Neuroscience, Aging & Stem Cell Research, Sanford-Burnham Medical Research Institute
* These authors contributed equally

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Inducerede pluripotente stamceller (iPSCs) repræsenterer en kilde til patient-specifikke væv til kliniske applikationer og grundforskning. Her præsenterer vi en detaljeret protokol til at omprogrammere humane perifere mononukleære celler (PBMNCs) opnået fra frosne buffy coats i virale-fri iPSCs anvender ikke-integrerer episomale plasmider.

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Developmental Biology Stem cellebiologi cellebiologi molekylærbiologi inducerede pluripotente stamceller perifere mononukleære celler omprogrammering episomale plasmider.
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