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建立临床相关<em>离体</em>调查上皮创伤修复的微环境本地人模拟白内障手术模型
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Developmental Biology
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JoVE Journal Developmental Biology
Establishment of a Clinically Relevant Ex Vivo Mock Cataract Surgery Model for Investigating Epithelial Wound Repair in a Native Microenvironment
Please note that all translations are automatically generated. Click here for the English version.

建立临床相关<em>离体</em>调查上皮创伤修复的微环境本地人模拟白内障手术模型

DOI:
10.3791/52886-v

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07:53 min

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June 05, 2015

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, , ,
1Pathology, Anatomy and Cell Biology,Thomas Jefferson University

Chapters

  • 00:05Title
  • 01:20Setup and Preparation of Lenses for Ex Vivo Wound Culture
  • 03:31Performing Mock Cataract Surgery
  • 04:43Preparing the Wounded Lens for Ex Vivo Culture
  • 05:35Separation of the Central Migration and Original Attachment Zone
  • 06:17Results: Injury Response of Cells in their Native Microenvironment
  • 07:18Conclusion

Summary

Automatic Translation

Automatic Translation

Described here is the establishment of a clinically relevant ex vivo mock cataract surgery model that can be used to investigate mechanisms of the injury response of epithelial tissues within their native microenvironment.

Tags

Keywords: Ex VivoCataract SurgeryEpithelial Wound RepairLens EpitheliumLens CapsuleLens Fiber CellsVimentinMesenchymal ProgenitorsWound Healing ResponseNative Microenvironment

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