Enriched Culture
Enriched Culture - Related Videos
09:35
Enrichment Cultures: Culturing Aerobic and Anaerobic Microbes on Selective and Differential Medias
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2023
Source: Christopher P. Corbo1, Jonathan F. Blaize1, Elizabeth Suter1 1 Department of Biological Sciences, Wagner College, 1 Campus Road, Staten Island NY, 10301 Prokaryotic cells are able to inhabit nearly every environment on this planet. As a kingdom, they possess a great metabolic diversity, allowing them to use a wide variety of molecules for energy generation (1). Therefore, when cultivating these organisms in the lab, all necessary and specific molecules required to make energy must be...
18:17
Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues
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Cited by 106 •
2011
This article describes methods to derive enriched populations of murine oligodendrocyte precursor cells (OPCs) in primary culture, which differentiate to produce mature oligodendrocytes (OLs). In addition, this report describes techniques to produce murine myelinating co-cultures by seeding mouse OPCs onto a neurite bed of mouse dorsal root ganglion neurons (DRGNs).
01:25
Isolation of Ammonium-Oxidizing Iron Reducers from a Wetland Soil–Derived Enrichment Culture
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2025
Begin with a wetland soil-derived enrichment culture containing bacterial communities.The culture exhibits Feammox activity, ammonium oxidation coupled to ferric iron reduction, indicating the enrichment of a bacterium capable of this metabolic process.Serially dilute the culture using media.Streak the diluted samples onto test agar plates supplemented with ferric iron and ammonium.Use control plates that lack either ferric iron or ammonium, or contain ferric iron with organic carbon instead of...
14:38
Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential
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Cited by 5 •
2012
Microbial eukaryotes are both a source of photosynthetically-derived carbon and top predatory species in permanently ice-covered Antarctic lakes. This report describes an enrichment culture approach to isolate metabolically versatile microbial eukaryotes from the Antarctic lake, Lake Bonney, and assesses inorganic carbon fixation potential using a radioisotope assay for Ribulose-1,5-bisphophate carboxylase oxygenase (RubisCO) activity.
09:13
Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures
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Cited by 6 •
2010
Here, we describe a method for efficient cryopreservation and thawing of cortical brain tissue blocks to generate highly enriched neuronal cultures. This simple protocol provides flexibility for later generation of neuronal, astrocyte, and neuronal precursor cell cultures.
FAQs
The added nutrients address metabolic requirements that a general medium may not meet. Carbon and nitrogen sources support energy production and cellular material, while vitamins, minerals, and other growth factors can assist essential cellular processes. By supplying these resources, the method increases the likelihood that nutritionally demanding microorganisms will replicate and become detectable in laboratory samples.
Each supplement supports a different aspect of cellular metabolism and replication. Carbon provides a metabolic resource, nitrogen contributes to cellular production, and vitamins or minerals can serve as required growth factors. The combined nutrient composition therefore influences which microorganisms can increase in biomass, making medium formulation important when researchers target organisms with particular nutritional requirements.
Controlled conditions create a consistent environment in which the supplied nutrients can support microbial growth and recovery. This helps researchers examine how microorganisms respond to the selected medium rather than relying on uncontrolled environmental variation. The resulting growth can then be used to recover organisms for further characterization, including studies of their ecological or biotechnological significance.
Environmental samples may contain microorganisms associated with nutrient cycling or pollutant transformation. Enrichment can increase the recovery and biomass of organisms with relevant nutritional requirements, making them more accessible for isolation and characterization. Researchers can then use the resulting cultures in microbial ecology studies or enrichment-based screening connected to environmental biotechnology and remediation strategies.
A typical workflow begins with a soil, sediment, or water sample and a nutrient-supplemented growth medium selected to support the microorganisms of interest. After growth under controlled conditions, researchers recover the organisms that increased in the culture and characterize them. This sequence links environmental sampling with laboratory-based investigation of microbial properties and potential functions.
Researchers choose this approach when they need to increase recovery or biomass of microorganisms that may be difficult to detect or grow effectively on a general medium. It is useful for isolating organisms from soil, sediment, and water, screening cultures for relevant traits, and supporting studies of microorganisms involved in nutrient cycling, pollutant transformation, or remediation.