22,703 Views
•
07:58 min
•
July 27, 2015
DOI:
Summary
Automatically generated
The isolated rat heart is an enduring model for ischemia reperfusion injury. Here, we describe the process of harvesting the beating heart from a rat via in situ aortic cannulation, Langendorff perfusion of the heart, simulated ischemia-reperfusion injury, and infarct staining to confirm the extent of ischemic insult.
Related Videos
10:25
Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury
Related Videos
17970 Views
15:07
Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle
Related Videos
15054 Views
11:12
In situ Transverse Rectus Abdominis Myocutaneous Flap: A Rat Model of Myocutaneous Ischemia Reperfusion Injury
Related Videos
13668 Views
09:09
Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice
Related Videos
39357 Views
07:07
Murine Model of Intestinal Ischemia-reperfusion Injury
Related Videos
19370 Views
06:32
Evaluation of Coronary Flow Reserve After Myocardial Ischemia Reperfusion in Rats
Related Videos
7814 Views
08:05
A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery
Related Videos
7207 Views
09:52
Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts
Related Videos
12769 Views
07:50
Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model
Related Videos
4551 Views
10:07
Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model
Related Videos
4960 Views
Read Article
Cite this Article
Herr, D. J., Aune, S. E., Menick, D. R. Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts. J. Vis. Exp. (101), e52908, doi:10.3791/52908 (2015).
Copy