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Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts
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Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts

Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts

DOI:
10.3791/52908-v

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07:58 min

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July 27, 2015

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, ,
1Department of Medicine/Cardiology, Gazes Cardiac Research Institute,Medical University of South Carolina, 2Ralph H. Johnson VA Medical Center,Medical University of South Carolina

Chapters

  • 00:05Title
  • 01:01Harvesting the Heart
  • 03:58Langendorff Perfusion and Ischemia Reperfusion Injury
  • 06:31Analysis of Ischemia Reperfusion Injured Hearts
  • 07:32Conclusion

Summary

Automatic Translation

Automatic Translation

The isolated rat heart is an enduring model for ischemia reperfusion injury. Here, we describe the process of harvesting the beating heart from a rat via in situ aortic cannulation, Langendorff perfusion of the heart, simulated ischemia-reperfusion injury, and infarct staining to confirm the extent of ischemic insult.

Tags

Keywords: Ischemia-reperfusion InjuryLangendorff-perfused Rat HeartsMyocardial InfarctionCardiac ComplicationsThoracic SurgeryPharmacological InterventionsIschemic PreconditioningLeft Ventricular PressureReperfusion InjuryBiochemical Events

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