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Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts
 

Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts

Article DOI: 10.3791/52908
July 27th, 2015

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Summary July 27th, 2015

The isolated rat heart is an enduring model for ischemia reperfusion injury. Here, we describe the process of harvesting the beating heart from a rat via in situ aortic cannulation, Langendorff perfusion of the heart, simulated ischemia-reperfusion injury, and infarct staining to confirm the extent of ischemic insult.

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