/

/

Quantitation of Endothelial Cell Adhesiveness In Vitro

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Quantitation of Endothelial Cell Adhesiveness In Vitro

Quantitation of Endothelial Cell Adhesiveness In Vitro

DOI:

10.3791/52924-v

•

10:24 min

•

June 18, 2015

•

Donna J. Lowe, Kenneth Raj

¹Biological Effects Department,Centre for Radiation, Chemicals and Environmental Hazards, Public Health England

Chapters

00:05Title
01:31Preparation of Glass Coverslips
02:41Preparation of Endothelial Cell Monolayer
04:18Preparation of Monocytes
06:50Enumeration of Adhesive Endothelial Cells
07:50Results: Quantification of Endothelial Cell Adhesiveness
09:37Conclusion

Summary

Automatic Translation

We report an in vitro method that allows the quantitation of the actual number of adhesive cells within an endothelial cell monolayer.

Tags

Keywords: Endothelial Cell Adhesiveness In Vitro Monocyte Adhesion Inflammation Adhesion Index Endothelial Monolayer Fluorescent Labeling Rinsing Procedure Cell Quantitation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

Quantitation of γH2AX Foci in Tissue Samples

NanoDrop Microvolume Quantitation of Nucleic Acids

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

In vitro Cell Migration and Invasion Assays

Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis

Analysis of Oxidative Stress in Zebrafish Embryos

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca²⁺]_iand Nitric Oxide Production

Read Article