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Implantation of Miniosmotic Pumps and Delivery of Tract Tracers to Study Brain Reorganization in Pathophysiological Conditions
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Implantation of Miniosmotic Pumps and Delivery of Tract Tracers to Study Brain Reorganization in Pathophysiological Conditions

Implantation of Miniosmotic Pumps and Delivery of Tract Tracers to Study Brain Reorganization in Pathophysiological Conditions

DOI:
10.3791/52932-v

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10:32 min

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January 18, 2016

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, , , ,
1Department of Neurology,University of Duisburg-Essen, 2Neuroscience Unit,Institute for Advanced Studies (IDEA), 3Department of Cell Biology,Simon Bolivar University

Chapters

  • 00:05Title
  • 01:11Preparation of Miniosmotic Pumps
  • 02:25Implantation of Miniosmotic Pumps
  • 05:03Removal of the Miniosmotic Pump
  • 05:57Pressure Tract Tracer Injection at 45o Angles on the Right Motor Cortex
  • 08:26Results: Recovery of Infarcted Brain After Delivery of Recombinant Human Erythropoietin
  • 09:40Conclusion

Summary

Automatic Translation

Automatic Translation

In order to study brain reorganization under pathological conditions we used miniosmotic pumps for direct protein delivery into the brain circumventing the blood brain barrier. Tract tracers are then injected to study alterations in brain connectivity under the influence of the protein.

Tags

Keywords: Mini-osmotic PumpsTract TracersBrain ReorganizationNeurorestorative ResearchLong Distance ConnectionsCentral Nervous SystemTherapeutic AgentsSurgical ProceduresStereotaxic FrameAnesthesiaSkull Exposure

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