Journal
/
Neuroscience
/
Visual Evoked Potential Recording in a Rat Model of Experimental Optic Nerve Demyelination
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Visual Evoked Potential Recording in a Rat Model of Experimental Optic Nerve Demyelination

Visual Evoked Potential Recording in a Rat Model of Experimental Optic Nerve Demyelination

DOI:
10.3791/52934-v

•

06:49 min

•

July 29, 2015

•

, , , , ,
1Department of Ophthalmology, Australian School of Advanced Medicine,Macquarie University, 2Save Sight Institute,The University of Sydney

Chapters

  • 00:05Title
  • 01:13VEP Electrode Implantation
  • 03:08Optic Nerve Injection
  • 04:15VEP Recording
  • 05:44Recordings and Histology
  • 06:17Conclusion

Summary

Automatic Translation

Automatic Translation

Focal demyelination is induced in the optic nerve using lysolecithin microinjection. Visual evoked potentials are recorded via skull electrodes implanted over the visual cortex to examine the signal conduction along the visual pathway in vivo. This protocol details the surgical procedures underlying electrode implantation and optic nerve microinjection.

Tags

Keywords: Visual Evoked PotentialVEPOptic NerveDemyelinationRodent ModelOptic NeuritisElectrodesLongitudinal AssessmentRemyelinationNeuroprotective Therapies

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52934?article_type=v

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System
check_url/52934?article_type=v

Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS
check_url/52934?article_type=v

Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
check_url/52934?article_type=v

Minimally-invasive Technique for Injection into Rat Optic Nerve
check_url/52934?article_type=v

Simultaneous Recording of Electroretinography and Visual Evoked Potentials in Anesthetized Rats
check_url/52934?article_type=v

In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments
check_url/52934?article_type=v

Partial Optic Nerve Transection in Rats: A Model Established with a New Operative Approach to Assess Secondary Degeneration of Retinal Ganglion Cells
check_url/52934?article_type=v

Visual Evoked Potential Recordings in Mice Using a Dry Non-invasive Multi-channel Scalp EEG Sensor
check_url/52934?article_type=v

Rat Model of Widespread Cerebral Cortical Demyelination Induced by an Intracerebral Injection of Pro-Inflammatory Cytokines
check_url/52934?article_type=v

Stimulus-specific Cortical Visual Evoked Potential Morphological Patterns

Read Article