Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel&#8217;s Cartilage

Lucie J. Rochard; Irving T.C. Ling; Yawei Kong; Eric C. Liao

doi:10.3791/52935

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Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage

9,049 Views

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06:40 min

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October 21, 2015

DOI:

10.3791/52935-v

DOI:

10.3791/52935-v

•

06:40 min

October 21, 2015

•

4 Views

Lucie J. Rochard*¹, Irving T.C. Ling*¹, Yawei Kong¹, Eric C. Liao¹

¹Massachusetts General Hospital, Center for Regenerative Medicine,Harvard Medical School

Summary

Cell organization of craniofacial bones has long been hypothesized but never directly visualized. Multi-spectral cell labeling and in vivo live imaging allows visualization of dynamic cell behavior in zebrafish lower jaw. Here, we detail the protocol to manipulate Zebrabow transgenic fish and directly observe cell intercalation and morphological changes of chondrocytes in the Meckel’s cartilage.