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Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage
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Developmental Biology
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JoVE Journal Developmental Biology
Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage

Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage

DOI:
10.3791/52935-v

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06:40 min

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October 21, 2015

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, , ,
1Massachusetts General Hospital, Center for Regenerative Medicine,Harvard Medical School

Chapters

  • 00:05Title
  • 01:21Embryo Collection, Tamoxifen Treatment, and Embryo Selection
  • 02:46Mounting Embryos in Methylcellulose and Fluorescence Microscopy
  • 04:33Results: Chondrocyte Intercalation and Directional Proliferation in ubi:Zebrabow-M;sox10:ERT2-Cre Transgenic Zebrafish
  • 06:15Conclusion

Summary

Automatic Translation

Automatic Translation

Cell organization of craniofacial bones has long been hypothesized but never directly visualized. Multi-spectral cell labeling and in vivo live imaging allows visualization of dynamic cell behavior in zebrafish lower jaw. Here, we detail the protocol to manipulate Zebrabow transgenic fish and directly observe cell intercalation and morphological changes of chondrocytes in the Meckel’s cartilage.

Tags

Keywords: ChondrocyteIntercalationProliferationZebrabowClonal Cell AnalysisMeckel’s CartilageCraniofacial DevelopmentCranial Neural Crest CellsCell MigrationCell AdhesionCell DifferentiationMandibular MorphogenesisFluorescent ProteinsConfocal Time-lapse ImagingMandibular Malformations

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