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Isolation and Primary Culture of Mouse Aortic Endothelial Cells
 

Isolation and Primary Culture of Mouse Aortic Endothelial Cells

Article DOI: 10.3791/52965-v 08:20 min December 19th, 2016
December 19th, 2016

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Summary

The vascular endothelial cells play a significant role in many important cardiovascular disorders. This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment. Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology.

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Keywords: Mouse Aortic Endothelial Cells Primary Culture Isolation Aorta Endothelium Heparin PBS Adipose Tissue Connective Tissue Endothelial Growth Medium Matrix Six-well Plate
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