Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

Yunfeng Chen; Baoyu Liu; Lining Ju; Jinsung Hong; Qinghua Ji; Wei Chen; Cheng Zhu

doi:10.3791/52975

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Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

12,419 Views

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14:09 min

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August 04, 2015

DOI:

10.3791/52975-v

DOI:

10.3791/52975-v

•

14:09 min

August 04, 2015

•

12398 Views

Yunfeng Chen*¹, Baoyu Liu*², Lining Ju*³, Jinsung Hong*¹, Qinghua Ji^4,5, Wei Chen⁶, Cheng Zhu¹

¹Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Biosciences,Georgia Institute of Technology ²Coulter Department of Biomedical Engineering,Georgia Institute of Technology ³Charles Perkins Centre,The University of Sydney ⁴Institute of Biophysics, Laboratory of RNA Biology,Chinese Academy of Sciences ⁵University of Chinese Academy of Sciences ⁶School of Medicine and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,Zhejiang University

Summary

We describe a technique for concurrently measuring force-regulated single receptor-ligand binding kinetics and real-time imaging of calcium signaling in a single T lymphocyte.