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Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes
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Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes

Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes

DOI:
10.3791/52982-v

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12:11 min

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August 27, 2015

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1Division of Cardiovascular Medicine, Department of Medicine,University of Massachusetts Medical School

Chapters

  • 00:05Title
  • 01:30Isolation of Primary Mouse Hepatocytes
  • 05:27Fatty Acid Oxidation Assay
  • 08:55Lipogenesis Assay
  • 10:13Results: Fatty Acid Oxidation and Lipogenic Responses to Respiration Altering Compounds
  • 11:33Conclusion

Summary

Automatic Translation

Automatic Translation

De novo lipogenesis and β-fatty acid oxidation constitute key metabolic pathways in hepatocyte, pathways that are perturbed in several metabolic disorders, including fatty liver disease. Here we demonstrate isolation of mouse primary hepatocytes and describe quantification of β-fatty acid oxidation and lipogenesis.

Tags

Fatty Acid OxidationLipogenesisPrimary Mouse HepatocytesLiver Lipid MetabolismBeta-oxidationDe Novo LipogenesisHepatic Lipid Handling

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