Liquid Scintillation Analysis
Liquid Scintillation Analysis - Related Videos
10:52
Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
0 Views •
Cited by 21 •
2016
Phosphoinositides are signaling lipids whose relative abundance rapidly changes in response to various stimuli. This article describes a method to measure the abundance of phosphoinositides by metabolically labeling cells with 3H-myo-inositol, followed by extraction and deacylation. Extracted glycero-inositides are then separated by high-performance liquid chromatography and quantified by flow scintillation.
09:21
Efficiency of Liquid-liquid Extraction
0 Views •
2023
Source: Kerry M. Dooley and Michael G. Benton, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA Liquid-liquid extraction (LLE) is a separation technique used instead of distillation when either: (a) the relative volatilities of the compounds to be separated are very similar; (b) one or more of the mixture components are temperature sensitive even near ambient conditions; (c) the distillation would require a very low pressure or a very high distillate/feed...
11:26
Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake
0 Views •
Cited by 20 •
2017
Synaptosomal dopamine uptake and high-performance liquid chromatography analysis represent experimental tools to investigate dopamine homeostasis in mice by assessing the function of the dopamine transporter and levels of dopamine in striatal tissue, respectively. Here we present protocols to measure dopamine tissue content and assess the functionality of the dopamine transporter.
09:00
High Throughput Analysis of Liquid Droplet Impacts
0 Views •
Cited by 3 •
2020
This protocol enables efficient collection of experimental high-speed images of liquid drop impacts, and speedy analysis of those data in batches. To streamline these processes, the method describes how to calibrate and set up apparatus, generate an appropriate data structure, and deploy an image analysis script.
06:04
Aqueous Humor as a Liquid Biopsy for Retinoblastoma: Clear Corneal Paracentesis and Genomic Analysis
0 Views •
Cited by 36 •
2021
The aqueous humor is a high-yield liquid biopsy for retinoblastoma, intraocular cancer that cannot be biopsied in vivo due to the risk of extraocular spread. Herein, a method for safely extracting aqueous humor via clear corneal paracentesis and steps for genomic analysis to identify prognostic biomarkers are presented.
FAQs
The solvent first receives energy from beta-particle interactions and then transfers that energy to fluor molecules in the cocktail. The fluor releases the energy as photons, creating light pulses that photomultiplier tubes can detect. This sequence links radionuclide emissions to an electronic signal, allowing the instrument to estimate radioactive activity from the measured pulse output.
Photomultiplier tubes detect the faint photons produced by fluor molecules and convert them into measurable electrical pulses. Their role is essential because the light generated in the cocktail represents the indirect signal from radioactive decay. Instrument software can then process these pulses as counts, providing a basis for estimating activity in a biological sample.
Quenching reduces or alters the detectable light produced after radionuclide emissions interact with the cocktail, while background signals contribute counts that are not attributable to the biological sample's intended radioactivity. Because both factors can distort the measured result, the instrument software accounts for them when converting light pulses into estimates of radioactive activity.
The biological sample is mixed with a liquid scintillation cocktail containing a solvent and fluor molecules. The prepared mixture is then measured by an instrument that detects light pulses with photomultiplier tubes. Software converts the recorded pulses into counts and estimates activity while accounting for quenching and background signals, producing data suitable for biological interpretation.
Researchers use this approach when they need sensitive measurements of radioactivity in biological samples. Supported applications include tracer studies, assays of DNA or protein synthesis, and analyses of metabolic activity. The resulting activity measurements can help follow labeled material or quantify radioactive signals associated with biological processes rather than relying only on qualitative observations.
Radioactive measurements can reveal how labeled material moves through a biological system, changes during metabolism, or is retained and lost over time. In this context, the method supports investigations of transport, turnover, and biochemical pathways. Comparing measured activity across samples or experimental conditions can help researchers examine the behavior of these processes in biological systems.