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Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium
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Neuroscience
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JoVE Journal Neuroscience
Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

DOI:
10.3791/53030-v

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06:48 min

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August 07, 2015

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, , ,
1Department of Biomedical Sciences,Seoul National University College of Medicine, 2FARB Laboratory, Biomedical Research Institute,Seoul National University Hospital, 3College of Life Sciences,Gwangju Institute of Science and Technology, 4Department of Ophthalmology,Seoul National University College of Medicine

Chapters

  • 00:05Title
  • 01:04Sub-retinal Injection of the Viral Vector
  • 03:50Evaluation of the Gene Delivery
  • 05:18Fluorescent Marker Expression in Transduced RPE Cells
  • 06:03Conclusion

Summary

Automatic Translation

Automatic Translation

Subretinal injection is a surgical technique for effective gene delivery to retinal pigment epithelium in the mouse eye. Here we describe an easy and replicable method for subretinal injection of viral vectors to retinal pigment epithelium in experimental mice.

Tags

Keywords: Gene TherapyViral VectorSubretinal InjectionRetinal Pigment EpitheliumRPEMiceOcular Gene TherapyIntravitreal InjectionFlat-mountGene Transduction

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