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Bioengineering

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Photopatterning Proteins and Cells in Aqueous Environment Using TiO2 Photocatalysis

Article DOI: 10.3791/53045
October 26th, 2015 •
1,2, 3, 3, 3, 2,4, 2,5, 6
1Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, 2CREST, Japan Science and Technology Agency, 3School of Fundamental Science and Engineering, Waseda University, 4Research Institute of Electrical Communication, Tohoku University, 5Graduate School of Biomedical Engineering, Tohoku University, 6Faculty of Science and Engineering, Waseda University

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October 26th, 2015

We describe a protocol for modifying cell affinity of a scaffold surface in aqueous environment. The method takes advantage of titanium dioxide photocatalysis to decompose organic film in the photo-irradiated region. We show that it can be used to create microdomains of scaffolding proteins, both ex situ and in situ.

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