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Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue

DOI:
10.3791/53058-v

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11:08 min

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September 05, 2015

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, , , ,
1Institute of Neurobiology,Heinrich Heine University Düsseldorf, 2Departments of Physiology and Neuroscience,New York University School of Medicine

Chapters

  • 00:05Title
  • 01:42Preparation of Double-barreled Ion-selective Microelectrodes
  • 04:36Preparation of Concentric Ion-selective Microelectrodes
  • 06:56Calibration of Ion-selective and Concentric Ion-selective Microelectrodes
  • 07:44Electrophysiology Experiments
  • 08:40Results: Calibration of Sodium-selective Microelectrodes and Comparison of Double-barreled Electrode with Concentric Electrode
  • 10:31Conclusion

Summary

Automatic Translation

Automatic Translation

We demonstrate the fabrication, calibration and properties of two types of ion-selective microelectrodes (double-barreled and concentric) for measurement of ion concentrations in brain tissue. These are then used in the mouse hippocampal slice preparation to show that excitatory activity changes both extracellular potassium and sodium concentrations.

Tags

Double-barreled MicroelectrodesConcentric MicroelectrodesExtracellular Ion SignalsPotassiumSodiumHippocampusAcute Brain SliceIon-selective Electrodes

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