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August 09, 2015
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Summary
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A method for surface-spreading chromosomes from budding yeast is presented. This method is derived from a method previously described by Loidl and Klein. In addition, we demonstrate a procedure for immunostaining of spread chromosomes.
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Cite this Article
Grubb, J., Brown, M. S., Bishop, D. K. Surface Spreading and Immunostaining of Yeast Chromosomes. J. Vis. Exp. (102), e53081, doi:10.3791/53081 (2015).
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