Surface Spreading and Immunostaining of Yeast Chromosomes

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Biology

JoVE Journal
Biology

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JoVE Journal Biology

Surface Spreading and Immunostaining of Yeast Chromosomes

DOI:

10.3791/53081-v

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12:06 min

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August 09, 2015

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Jennifer Grubb, M. Scott Brown², Douglas K. Bishop²

¹Department of Radiation and Cellular Oncology,University of Chicago, ²Department of Molecular Genetics and Cell Biology,University of Chicago

Chapters

00:05Title
01:56Preparation of Spheroplasts
03:51Chromosome Spreading
06:07Immunostaining
09:36Results: Optimal Chromosome Spreading with and without the Use of Lipsol
11:28Conclusion

Summary

Automatic Translation

A method for surface-spreading chromosomes from budding yeast is presented. This method is derived from a method previously described by Loidl and Klein. In addition, we demonstrate a procedure for immunostaining of spread chromosomes.

Surface Spreading and Immunostaining of Yeast Chromosomes

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Chapters

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