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Using Caco-2 Cells to Study Lipid Transport by the Intestine

Article DOI: 10.3791/53086
August 20th, 2015 •
1, 2
1Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, 2Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University

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August 20th, 2015

Caco-2 cells can serve as an in vitro model to study the enterocyte transport of lipids, and lipid-soluble drugs/vitamins. The permeable membrane system separates the apical from the basolateral compartment, while the lentivirus expression system offers an effective gene overexpression method. The isolation of lipoproteins is confirmed by TEM.

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