Intestinal Lipoprotein Isolation
Intestinal Lipoprotein Isolation - Related Videos
10:39
Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification
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Cited by 40 •
2016
This protocol describes the isolation and quantification of high-density lipoprotein small RNAs.
09:48
Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein
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Cited by 2 •
2016
MicroRNAs play an important regulatory role and are emerging as novel therapeutic targets for various human diseases. It has been shown that miRNAs are carried in high density lipoproteins. We have developed a simplified method to rapidly isolate purified HDL suitable for miRNA analysis from human plasma.
04:37
Intestinal Crypts Isolation: A Method to Isolate Whole Crypts from Small Intestine of Murine Model
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2023
In this video, we demonstrate a technique to isolate whole crypts from a mouse’s small intestine. The isolated whole crypts can be used to propagate long-lived and self-renewing 3D organoids in vitro.
06:47
Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
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Cited by 9 •
2021
Several methods have been used for analyzing plasma lipoproteins; however, ultracentrifugation is still one of the most popular and reliable methods. Here, we describe a method regarding how to isolate lipoproteins from plasma using sequential density ultracentrifugation and how to analyze the apolipoproteins for both diagnostic and research purposes.
04:16
Enrichment of Bacterial Lipoproteins Using Non-Ionic Detergent Phase Separation
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2025
Source: Armbruster, K. M., Meredith, T. C. Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry. J. Vis. Exp. (2018)This video demonstrates the enrichment of bacterial lipoproteins using a non-ionic detergent that undergoes phase separation upon warming. Through sequential temperature shifts and centrifugation steps, lipoproteins are selectively partitioned into the detergent phase, while non-lipoprotein contaminants...
FAQs
Density-based fractionation separates particles according to buoyant density, allowing intestinal lipoprotein populations to occupy different fractions during ultracentrifugation. Differences in particle composition contribute to these density relationships. Examining the recovered fractions helps investigators distinguish chylomicrons from other lipoproteins and evaluate how intestinal lipid transport is organized.
Buoyant density provides the physical basis for separating lipid-carrying particles that differ in composition and particle properties. This makes it possible to recover fractions enriched in particular lipoprotein populations rather than analyzing the intestinal material as one mixture. The resulting separation supports comparisons of particle distribution and transport-related changes.
Recovered fractions can be examined for lipid content, apolipoprotein content, particle distribution, and associated enzymes. Together, these measurements provide more than a particle count: they reveal compositional differences and biochemical associations among intestinal lipoprotein populations. Such profiles can help relate particle characteristics to lipid transport and lipoprotein processing.
A typical workflow begins with intestinal samples or lymph, followed by preparation under controlled conditions. The material is then subjected to density-based fractionation, often using ultracentrifugation, to separate particles into fractions. Investigators recover those fractions and characterize their lipids, apolipoproteins, particle distribution, or associated enzymes to address the experimental question.
Controlled preparation provides a consistent starting material for density-based separation and downstream characterization. Because the analysis compares recovered fractions, variation introduced before ultracentrifugation could affect apparent particle distribution or composition. Maintaining consistent preparation therefore supports more reliable interpretation of lipid, apolipoprotein, and enzyme measurements across samples or experimental groups.
This method is useful when researchers need to investigate dietary fat absorption, intestinal lipoprotein assembly, or transport of lipids from the intestine. It can also reveal how experimental treatments alter particle composition or distribution. By linking fraction characteristics with transport functions, the approach supports mechanistic studies of intestinal lipid handling.
Comparing intestinal lipoprotein fractions between experimental conditions can identify changes associated with metabolic disease or a treatment. Researchers may assess whether particle distribution, lipid content, apolipoprotein content, or associated enzymes differ between groups. These measurements provide a biochemical basis for examining altered intestinal lipid transport rather than relying only on overall sample composition.