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Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability
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Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability

Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability

DOI:
10.3791/53106-v

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09:11 min

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February 23, 2016

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, , , , , , , ,
1Wolfson Centre for Age-Related Diseases,King’s College London, University of London, 2Department of Neuroimaging, James Black Centre, Institute of Psychiatry,King’s College London, University of London, 3Institute of Neuroscience and Psychology, Wellcome Surgical Institute, College of Medical, Veterinary and Life Sciences,University of Glasgow, Glasgow, 4Research Service,Edward Hines Jr. VA Hospital, 5Neurology Service,Edward Hines Jr. VA Hospital, 6Department of Molecular Pharmacology and Therapeutics, Neuroscience Research Institute,Loyola University Chicago, 7Department of Oncology, The Gray Institute for Radiation, Oncology and Biology,University of Oxford

Chapters

  • 00:05Title
  • 01:03Stroke Surgery
  • 06:48Confirmation of Infarct and Sample Size Calculation
  • 07:39Results: Representative Micro and Macroanatomical Effects of MCA Occlusion on Cerebral Function in the Elderly Rat
  • 08:32Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol to produce permanent distal middle cerebral artery occlusion in elderly female rats with simultaneous occlusion of the carotid arteries to generate large cortical infarcts and sustained deficits. We show confirmation of the lesion size using structural MRI at 24 hr and 8 weeks after stroke.

Tags

Keywords: Permanent Middle Cerebral Artery OcclusionCarotid Artery OcclusionElderly Rat ModelCortical IschemiaSustained DisabilityMRIInfarctBehavioral DeficitsSurgical ProcedureCommon Carotid ArteryVagus NerveCortical IschemiaStroke Model

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