Visible-light Induced Reduction of Graphene Oxide Using Plasmonic Nanoparticle

The overall goal of this synthetic method to produce reduced graphene oxide using visible light with plasmonic nanoparticles at room temperature is to give a simple chemical free and energy efficient method for reduced graphene oxide preparation. This method can help answer key questions in the plasmonic nanoparticle assisted photo reduction of graphine oxide field, such as how the reaction progress has strong dependence on the structure of plasmonic nanoparticles, including cervical gold nanoparticles, gold nano rods, and gold nano stars. The main advantage of this technique is the use of visible light with plasmonic nanoparticles for reduced graphine oxide preparation, while shows excellent quenching properties over the fluorescence molecules modified on S-S-D-N-A and excellent fluorescence recovery.

We lyophilize the graphene oxide solution to produce a fluffy graphene oxide powder to make the nano-sized graphene oxide solution dissolve 20 milligrams of graphene oxide powder in 40 milliliters of triple distilled water, then exfoliate by prolonged sonication until the entire size distribution becomes below 150 nanometers. Next, centrifuge the solution two times to remove the Unex exfoliated large graphene oxide sheet precipitates to prepare gold nano rods. First formulate the seed solution by adding a freshly prepared 0.6 milliliter ice cold solution of sodium borohydride into an aqueous mixture of 0.25 milliliters of chloro orric acid and 9.75 milliliters of CE trimethyl ammonium bromide.

Stir the resulting mixture vigorously for 30 seconds and keep it at 28 degrees Celsius for three hours. Prepare the growth solution by mixing 475 milliliters of C ttab, three milliliters of silver nitrate and 20 milliliters of chloro oric acid. Then add 3.2 milliliters of freshly prepared ascorbic acid to the mixture, followed by the addition of 0.8 milliliters of an aqueous hydrochloric acid solution.

In the final step, add 3.2 milliliters of seed solution to the growth solution at 28 degrees Celsius and subject the reaction mixture to quick inversion. For a few seconds following inversion, keep the resulting mixture undisturbed for at least six hours. Analyze the prepared gold nano rods with ultraviolet visible spectroscopy for absorption maxima at 730 nanometers and with transmission electron microscopy analysis for an aspect ratio confirmation of 3.5.

To prepare gold nano stars, mix 20 milliliters of phosphate buffer with 30 milliliters of heaps buffer. Then add 500 microliters of 20 millimolar hydrogen tetra chloro array to the mixture and keep at 28.5 degrees Celsius for 30 minutes in a water bath solution, color change from light yellow to greenish blue can be observed after 30 minutes. Next, centrifuge the solution at 8, 928 times G for 30 minutes and disperse the precipitates in distilled water.

Finally, analyze the prepared gold nano stars with ultraviolet visible spectroscopy for absorption maxima at 740 nanometers and with transmission electron microscopy analysis for particle size, confirmation of 30 nanometers to prepare reduced graphene oxide. First, add one milliliter of plasmonic nanoparticles and 100 microliters of ammonium hydroxide to 10 milliliters of graphene oxide solution. Placed in a Pyrex glass reactor equipped with a water circulating jacket, irradiate the mixture with a xenon lamp for 30 minutes with water circulation through a water circulating jacket to maintain the temperature at 25 degrees Celsius.

Then centrifuge the solution at 10, 625 times G for 15 minutes. To remove the gold nanoparticles, take the supernatant containing the prepared reduced graphene oxide to analyze on an ultraviolet visible spectrophotometer in the range of 200 to 900 nanometers. The structural changes in the reduced graphene oxide were analyzed by x-ray diffraction Disappearance of the graphene oxide peak at 10.2 clearly indicated the formation of reduced graphene oxide.

The D peak to G peak intensity ratios were measured by ramen analysis for both graphene oxide and reduced graphene oxide produced by a chemical method or a light induced method with or without nanoparticles. To qualitatively demonstrate the structural changes in the reduced graphene oxide shown here are the summarized fluorescent emission spectra of SI three modified DNA after incubating with graphene oxide or reduced graphene oxide solutions. The decreased intensity indicates the quenching efficiency of graphene oxide and reduced graphene oxide.

The reduced graphene prepared with gold nanoparticles and visible light showed the most efficient quenching efficiency. The results indicate that the reduced graphene oxide prepared using visible light and plasmonic nanoparticles have comparable physical properties to chemically reduced graphene oxide. After watching this video, you should have a good understanding of how to prepare the ate peroxide by a simple method using plasma nanoparticle and the visible light.

Once master, this technique can be done in 30 minutes if it is performed the properly while attempting this procedure. It's important to remember IDE synthesis step using human method and the REOC LI step using visible light and the nanoparticles After its development. This technique paved the wave for researchers in the field of graphine oxide reduction to explore the effect of plasmonic nanoparticles in visible light.

I don't forget that working with concentrated sulfuric acid and phosphoric acid can be extremely hazardous and precautions such as using fume hood, wearing hand gloves and mask should always be taken while performing this procedure.