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Investigating Functional Regeneration in Organotypic Spinal Cord Co-cultures Grown on Multi-electrode Arrays
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Investigating Functional Regeneration in Organotypic Spinal Cord Co-cultures Grown on Multi-electrode Arrays

Investigating Functional Regeneration in Organotypic Spinal Cord Co-cultures Grown on Multi-electrode Arrays

DOI:
10.3791/53121-v

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08:25 min

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September 23, 2015

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, ,
1Department of Physiology,University of Bern

Chapters

  • 00:05Title
  • 01:25Preparation of Multi-Electrode Arrays
  • 02:39Spinal Cord Tissue Dissection
  • 03:52Mounting Spinal Cord Tissue Slices on Multi-Electrode Arrays
  • 05:09Mechanical Lesions
  • 06:11Electrophysiological Recordings of Spontaneous Activity
  • 06:59Results: Synchronized Activity in Slices is Reduced with Age
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol that is based on spinal cord slices cultured on multi-electrode arrays to study functional regeneration of propriospinal connections in vitro.

Tags

Organotypic Spinal Cord Co-culturesMulti-electrode ArraysFunctional RegenerationPropriospinal ConnectionsSpinal Cord InjuryAdult Higher VertebratesIn Vitro ModelNetwork ActivitySynchronized BurstsImmunohistochemical Stainings

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