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Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
Chapters
Summary October 31st, 2015
Preparation of protein-functionalized planar glass-supported lipid bilayers, determination of protein mobility within and measurement of protein densities is shown here. A roadmap to building a noise-reduced Total Internal Reflection microscope is outlined, which allows visualizing single bilayer-resident fluorochromes with high spatiotemporal resolution.
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