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Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

DOI:
10.3791/53158-v

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20:00 min

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October 31, 2015

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, ,
1Institute of Applied Physics – Biophysics,Vienna University of Technology, 2Institute for Hygiene and Applied Immunology, Immune Recognition Unit,Medical University of Vienna

Chapters

  • 00:00Title
  • 00:23Introduction
  • 01:36Vesicle Preparation
  • 04:22Bilayer Preparation
  • 08:12Principles of Total Internal Reflection Microscopy
  • 10:44Architectural of the Microscopy System
  • 12:41Power Measurement
  • 15:38Measurement Bilayer Fluidity
  • 16:39Measurement Protein Density on the Bilayer
  • 19:25Conclusion

Summary

Automatic Translation

Automatic Translation

Preparation of protein-functionalized planar glass-supported lipid bilayers, determination of protein mobility within and measurement of protein densities is shown here. A roadmap to building a noise-reduced Total Internal Reflection microscope is outlined, which allows visualizing single bilayer-resident fluorochromes with high spatiotemporal resolution.

Tags

Single Molecule MicroscopyPlanar Supported BilayersLipid BilayersSingle Molecule ImagingProtein DynamicsTIRF MicroscopyCell MembraneCell-cell ContactEndocytosisExocytosisImmune Recognition

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