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JoVE Journal
Bioengineering
Author Produced

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Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
 

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Article DOI: 10.3791/53158
October 31st, 2015

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Summary October 31st, 2015

Preparation of protein-functionalized planar glass-supported lipid bilayers, determination of protein mobility within and measurement of protein densities is shown here. A roadmap to building a noise-reduced Total Internal Reflection microscope is outlined, which allows visualizing single bilayer-resident fluorochromes with high spatiotemporal resolution.

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