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JoVE Journal Neuroscience
Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome
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Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

Article DOI: 10.3791/53165-v • 08:49 min • September 23rd, 2015
September 23rd, 2015 •
1, 1, 2, 2
1Ross University School of Veterinary Medicine, 2Charles E. Schmidt College of Medicine, Florida Atlantic University

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Summary
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This protocol describes a rapid and simplified in situ hybridization method ideal forparaformaldehyde-prefixed brain, thus reducing the need for prolonged complex steps while using fresh frozen tissues. The method is validated using the identification of the serotonin 5-HT2A receptor gene htr2a in rats.

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Rapid In Situ Hybridization Oligonucleotide Probes Paraformaldehyde-prefixed Brain Rats Serotonin Syndrome MDMA Toxicity Serotonergic MRNA Expression Serotonin 5-HT2A Receptor Gene Htr2a Radioactive Riboprobe Fresh Frozen Tissues RNase-free Environment Short Oligonucleotide Probes Prefixed Brain Sections Hybridization Probes DapB PpiB Htr2a Non-radioactive Oligonucleotide Probes
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