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Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart
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Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart

Article DOI: 10.3791/53166-v • 09:26 min • September 10th, 2015
September 10th, 2015 •
1, 2, 1
1Department of Pharmacology, University of California Davis, 2Departments of Pharmacology and Biomedical Engineering, University of California Davis

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This article describes the detailed protocol and equipment necessary for dual optical mapping of transmembrane potential (Vm) and free intra-sarcoplasmic reticulum (SR) Ca2+ in the Langendorff-perfused rabbit heart. This method allows for direct observation and quantification of Vm and SR Ca2+ dynamics in the intact heart.

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Optical Mapping Intra-Sarcoplasmic Reticulum Ca2+ Transmembrane Potential Langendorff-perfused Rabbit Heart SR Ca2+ Handling Excitation-contraction Coupling Arrhythmia Tissue Level Investigation Intact Heart Dual Optical Mapping Fluo-5N Low-affinity Ca2+ Indicator Cytosol Fluorescence SR Lumen Fluorescence Millimolar Range Epicardial Surface Bidirectional Relationship Arrhythmogenic Phenomena
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