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Biology

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Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
 

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Article DOI: 10.3791/53179
December 1st, 2015

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Summary December 1st, 2015

The indirect immunofluorescence protocol described in this article allows the detection and the localization of proteins in the mouse mammary gland. A complete method is given to prepare mammary gland samples, to perform immunohistochemistry, to image the tissue sections by fluorescence microscopy, and to reconstruct images.

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