Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery

The overall goal of the following experiment is to enable repeated measurements of the hydraulic conductivity or water permeability of the walls of Ular microvessels in the rat mesentary under controlled experimental conditions. This is achieved by cannulating a ular micro vessels to enable the oncotic and hydrostatic pressures in the lumen to be set and to allow the chemical composition of the profuse eight and Suses eight to be precisely controlled. Next, the vessel is occluded and red cells suspended in the PERFUSE eight, which act as flow markers can be observed moving in the lumen as indicators of trans vascular fluid exchange.

Then the position and velocity of the marker red cells are recorded either towards the site of occlusion or away from the occlusion site. Finally, occlusion is released and repeated for additional measurements or the vessel is rec. Cannulated and measurements are repeated under changed conditions.