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Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

DOI:
10.3791/53225-v

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09:32 min

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November 11, 2015

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, , , ,
1Department of Cardiology,Keio University School of Medicine, 2Department of Emergency and Critical Care Medicine,Keio University School of Medicine

Chapters

  • 00:05Title
  • 01:19Preparation of Activated Human T Cells
  • 04:05Generating Induced Pluripotent Stem Cells (iPSCs) from Activated T Cells
  • 07:11Expansion of T Cell-derived iPSCs
  • 08:28Results: Generation of iPSCs from Human Peripheral T Cells
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to generate induced pluripotent stem cells (iPSCs) from human peripheral T cells in feeder-free conditions using a combination of matrigel and Sendai virus vectors containing reprogramming factors.

Tags

Keywords: Induced Pluripotent Stem CellsIPSCsPeripheral T CellsSendai VirusFeeder-free ConditionsRegenerative TherapiesT Cell ReceptorAntigen-specific T CellsGenome RearrangementClinical ApplicationFetal Bovine SerumFeeder CellsBiosafetyActivated T Cells

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