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Immunology and Infection
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Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
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Immunology and Infection
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JoVE Journal Immunology and Infection
Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

DOI:
10.3791/53243-v

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07:47 min

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January 01, 2016

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, , ,
1Department of Biochemistry,Yong Loo Lin School of Medicine, 2Department of Chemistry,University of Utah, 3Synthetic Biology Research Consortium,National University of Singapore

Chapters

  • 00:05Title
  • 00:44Crystal Violet Quantitation of Biofilm Formation in A. baumannii S1
  • 02:48Confocal Laser Scanning Microscopy A. baumannii S1 Biofilms
  • 05:00Results: Pathogenic Biofilm Disruption
  • 06:28Conclusion

Summary

Automatic Translation

Automatic Translation

Quorum-quenching enzymes are anti-virulent and anti-bacterial options that can mitigate pathogenesis without risk of incurring resistance, by preventing the expression of virulence factors and genes associated with antibiotic resistance and biofilm formation. In this study, we report a method that demonstrates the efficacy of quorum-quenching enzymes in bacterial biofilm disruption.

Tags

Keywords: Quorum-quenchingLactonasesAnti-bacterialBiofilm DisruptionA.baumanniiCrystal VioletConfocal Laser Scanning MicroscopyCLSM

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