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Biology

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High Throughput Danio Rerio Energy Expenditure Assay
 

High Throughput Danio Rerio Energy Expenditure Assay

Article DOI: 10.3791/53297 08:35 min January 27th, 2016
January 27th, 2016

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Summary

Zebrafish are an important model organism for the study of energy homeostasis. By utilizing a NADH2 sensitive redox indicator, alamar Blue, we have developed an assay that measures the metabolic rate of zebrafish larvae in a 96 well plate format and can be applied to drug or gene discovery.

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High Throughput Danio Rerio Energy Expenditure Assay Resazurin-based Fluorometric Assay Genetic Manipulation Pharmacological Manipulation NADH2 Production Metabolic Rate Obesity Field Drug-gene Interactions Whole Animal System Oxygen Consumption Assay Small Differences In Metabolic Rate Caroline Foy E3 Medium Methylene Blue Flame Polish Disposable Pipette Zebrafish Crossings Egg Collection
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